Graduate School at Shenzhen, Tsinghua University
3D Cultured Mesenchymal Stem Cells: Improved Trafficking Ability and Therapeutic Effect on Strokes
Mesenchymal stem cells (MSC) have shown profound therapeutic potential in tissue repair/regeneration, and intravenous infusion has been a popular transplantation route of the cells for its convenience and safety. However, MSC are largely entrapped in lungs after intravenous delivery and die shortly, resulting in very long targeted engraftment into injured tissues. We have shown that this defect of MSC is largely caused by the enlargement and upregulation of integrins in expression and activation during culture expansion in monolayers (2D); notably, 3D spheroid culture could dramatically decrease the size of MSC and the expression levels of integrins, leading to increased MSC trafficking ability in the blood circulation, homing and engraftment efficiency to the ischemic brain, and ultimately therapeutic efficacy for ischemic strokes.
Dr. Yaojiong Wu is the professor of Graduate School at Shenzhen, Tsinghua University and professor of Precision Medicine and Healthcare Research Center
at Tsinghua-Berkeley Shenzhen Institute. His research focuses on the Niches for stem cell self-renewal, regeneration and organogenesis, epigenetic regulations of stem cell self-renewal, mesenchymal stem cell trafficking, homing and tissue repair/regeneration and bioengineering of the skin and hair follicle neogenesis.